Human Glutamate [NMDA] receptor subunit zeta-1(GRIN1) ELISA kit

Mfr.No.

CSB-EL009911HU

Alternative Names

glutamate receptor, ionotropic, N-methyl D-aspartate 1, NMDA1, NMDAR1, NR1, N-methyl-D-aspartate receptor channel, subunit zeta-1, NMDA receptor 1, glutamate (NMDA) receptor subunit zeta 1

Reactivity

Human

Detection Range

125 pg/mL-8000 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human ?-Glucosidase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	85-101
EDTA plasma (n=4)	93	87-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ?-Glucosidase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:20	Average %	95
	Range %	85-103
1:40	Average %	90
	Range %	85-99
1:80	Average %	95
	Range %	91-102
1:100	Average %	89
	Range %	81-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

µU/ml	OD1	OD2	Average	Corrected
200	2.127	2.112	2.120	1.913
100	1.746	1.757	1.752	1.545
50	1.442	1.417	1.430	1.223
25	1.069	1.028	1.049	0.842
12.5	0.835	0.843	0.839	0.632
6.25	0.502	0.521	0.512	0.305
3.12	0.347	0.354	0.351	0.144
0	0.205	0.209	0.207	?

Species

Homo sapiens

Sensitivity

31.25 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q05586
Antigen	glutamate receptor, ionotropic, N-methyl D-aspartate 1

Applications

ELISA

Research Area

GRIN1

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502519	CSB-EL009911HU	96T	$695	Add to Cart
BHE10502519	CSB-EL009911HU	5x96T	$3336	Add to Cart
BHE10502519	CSB-EL009911HU	10x96T	$6602.50	Add to Cart

Human Glutamate [NMDA] receptor subunit zeta-1(GRIN1) ELISA kit

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