Human Glutamate Carboxypeptidase 2

Mfr.No.

E5334Hu

Sensitivity

1.22ng/mL

Detection Range

2-600ng/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Cell growth-inhibiting gene 27 protein;FGCP;Folate hydrolase 1;FOLH 1;FOLH1;Folylpoly-gamma-glutamate carboxypeptidase;GCPII;Glutamate carboxypeptidase 2;Glutamate carboxypeptidase II;Membrane glutamate carboxypeptidase;mGCP;NAALADase I;N-acetylated-alpha-linked acidic dipeptidase I;Prostate-specific membrane antigen;PSM;PSMA;Pteroylpoly-gamma-glutamate carboxypeptidase

Molecular Weight

84331

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5334Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	39.4	2.18	5.5
Intra-Assay	2	18	175.5	6.66	3.8
Intra-Assay	3	18	137.2	7.95	5.8

Background

Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate Peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Involved in prostate tumor progression. Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.

Protocol

These standard curves of E5334Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
320ng/mL	2.253	2.252	2.203
	2.25
160ng/mL	1.427	1.423	1.375
	1.419
80ng/mL	0.851	0.79	0.741
	0.729
40ng/mL	0.453	0.485	0.436
	0.517
20ng/mL	0.29	0.271	0.222
	0.252
0ng/mL	0.043	0.049	0
	0.054

Gene ID	2346
UniProt ID	Q04609

Research Area	Metabolism

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106697	E5334Hu	48T	$320		Add to Cart
BHE12106697	E5334Hu	96T	$458		Add to Cart

Human Glutamate Carboxypeptidase 2 - FOLH1 ELISA Kit

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