Human Glucose-6-phosphate Isomerase

Mfr.No.

E0887Hu

Description

This sandwich kit is for the accurate quantitative detection of human Glucose-6-phosphate Isomerase (also known as GPI) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

AMF;Autocrine motility factor;Glucose-6-phosphate isomerase;GPI;Neuroleukin;NLK;PGI;PHI;Phosphoglucose isomerase;Phosphohexose isomerase;SA-36;Sperm antigen 36

Reactivity

Human

Detection Range

7-1500ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

63147

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

3.51ng/ml

Background

In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis (PubMed: 28803808). Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility (PubMed: 11437381). Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons (PubMed: 3352745, PubMed: 11004567). It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion (PubMed: 3352745, PubMed: 11004567).

Gene ID	2821
UniProt ID	P06744
Antigen	GPI

Research Area

Metabolism

Protocol

These standard curves of E0887Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/ml	2.269	2.322	2.27
	2.376
400ng/ml	1.789	1.749	1.696
	1.708
200ng/ml	1.163	1.23	1.178
	1.297
100ng/ml	0.604	0.666	0.613
	0.727
50ng/ml	0.394	0.397	0.345
	0.401
0ng/ml	0.056	0.052	0
	0.048

Precision

We measured random samples of E0887Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	185.3	12.8	6.9
Intra-Assay	2	18	321.9	11.83	3.7
Intra-Assay	3	18	45.9	2.79	6.1

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102562	E0887Hu	48T	$320		Add to Cart
BHE12102562	E0887Hu	96T	$458		Add to Cart

Human Glucose-6-phosphate Isomerase - GPI ELISA Kit

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