Human Glucose-6-phosphatase - G-6-PASE ELISA Kit

Mfr.No.

E5332Hu

Sensitivity

0.15U/mL

Detection Range

0.3-90U/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

G 6PC;G6Pase;G-6-Pase;G6Pase-alpha;G6PC;Glucose-6-phosphatase;Glucose-6-phosphatase alpha

Molecular Weight

40484

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5332Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4.4	0.27	6
Intra-Assay	2	18	15.7	0.85	5.4
Intra-Assay	3	18	34.9	1.55	4.4

Background

Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production in the terminal step of glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels.

Protocol

These standard curves of E5332Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48U/mL	2.328	2.202	2.141
	2.075
24U/mL	1.382	1.379	1.318
	1.376
12U/mL	0.831	0.825	0.765
	0.819
6U/mL	0.441	0.488	0.427
	0.535
3U/mL	0.298	0.274	0.213
	0.249
0U/mL	0.062	0.061	0
	0.059

Gene ID	2538
UniProt ID	P35575

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106695	E5332Hu	48T	$320		Add to Cart
BHE12106695	E5332Hu	96T	$458		Add to Cart

Human Glucose-6-phosphatase - G-6-PASE ELISA Kit

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