Human Glucokinase Regulatory Protein

Mfr.No.

E1470Hu

Sensitivity

0.016ng/mL

Detection Range

0.05-15ng/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

FGQTL5;GCKR;GKRP;Glucokinase regulator;Glucokinase regulatory protein

Molecular Weight

68685

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1470Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2.69	0.16	5.86
Intra-Assay	2	18	4.57	0.17	3.75
Intra-Assay	3	18	1.26	0.06	4.75

Background

The glucokinase regulatory protein (GKRP) also known as glucokinase (hexokinase 4) regulator (GCKR) is a protein produced in hepatocytes (liver cells). GKRP binds and moves glucokinase (GK), thereby controlling both activity and intracellular location of this key enzyme of glucose metabolism.

Protocol

These standard curves of E1470Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/mL	2.413	2.504	2.446
	2.595
4ng/mL	1.597	1.582	1.523
	1.566
2ng/mL	0.9	0.81	0.751
	0.72
1ng/mL	0.378	0.396	0.337
	0.414
0.5ng/mL	0.247	0.255	0.196
	0.262
0ng/mL	0.052	0.058	0
	0.064

Gene ID	2646
UniProt ID	Q14397

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103087	E1470Hu	48T	$320		Add to Cart
BHE12103087	E1470Hu	96T	$458		Add to Cart

Human Glucokinase Regulatory Protein - GCKR ELISA Kit

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