Human Glucocorticoid receptor(NR3C1) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL016059HU

Alternative Names

nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor), GCCR, GCR, GR, GRL, glucocorticoid receptor

Reactivity

Human

Detection Range

31.25 pg/mL-2000 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of pig TCC C5b-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	88-109
EDTA plasma (n=4)	97	85-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig TCC C5b-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	99
	Range %	85-105
1:2	Average %	95
	Range %	85-113
1:4	Average %	95
	Range %	82-102
1:8	Average %	97
	Range %	85-110

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

7.81 pg/mL

Limit Of Detection

450 nm

UniProt ID	P04150
Antigen	nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)

Applications

ELISA

Research Area

NR3C1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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