Human glucagon-like peptide-1,GLP-1 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08119h

Sensitivity

1.4 ng/mL

Detection Range

2.3 ng/mL-40 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

glucagon-like peptide-1,GLP-1

Recovery

The recovery of rat DHT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-95
EDTA plasma (n=4)	102	97-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat DHT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	102
	Range %	98-106
1:2	Average %	93
	Range %	89-97
1:4	Average %	95
	Range %	89-99
1:8	Average %	102
	Range %	98-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	GLP-1

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Extra virgin olive oil use is associated with improved post-prandial blood glucose and LDL cholesterol in healthy subjects Violi F. et al,Nutr Diabetes,2015 Jul 20;5:e172. Effect of Coenzyme Q10 on the Incidence of Atrial Fibrillation in Patients With Heart Failure. Zhao Q et al,J Investig Med,2015 Jun;63(5):735-9 Purslane (Portulaca oleracea) Seed Consumption And Aerobic Training Improves Biomarkers Associated with Atherosclerosis in Women with Type 2 Diabetes (T2D) F Dehghan.et al,Scientific Reports ,Dec 5 Acute effect of smoking and smoking abstinence on energy intake and appetite-related hormones blood concentrations Yannakoulia M.et al,Physiol Behav,Nov 8 Electroacupuncture for functional dyspepsia and the influence on serum Ghrelin, CGRP and GLP-1 levels QIANGLiming.et al,World Journal of Acupuncture-Moxibustion,June 26

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