Human Glial Fibrillary Acidic Protein

Mfr.No.

E2094Hu

Sensitivity

0.026ng/ml

Detection Range

0.05-15ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Glial Fibrillary Acidic Protein (also known as GFAP) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

GFAP;Glial fibrillary acidic protein

Molecular Weight

49880

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E2094Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2.8	0.16	5.9
Intra-Assay	2	18	5.9	0.18	3.1
Intra-Assay	3	18	4.2	0.2	4.8

Background

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Protocol

These standard curves of E2094Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	1.731	1.8	1.761
	1.87
4ng/ml	0.883	0.916	0.877
	0.95
2ng/ml	0.531	0.544	0.505
	0.558
1ng/ml	0.278	0.24	0.201
	0.202
0.5ng/ml	0.122	0.132	0.092
	0.141
0ng/ml	0.031	0.04	0
	0.048

Gene ID	2670
UniProt ID	P14136

Research Area	Neuroscience

Storage	2-8°C

Reference	S100 Calcium-Binding Protein B and Glial Fibrillary Acidic Protein in Patients with Mild Traumatic Brain Injury Joual: Bull Emerg Trauma. 2021 Oct;9(4):183-187. The Effect of Biomarkers and Optic Nerve Sheath Diameter in Determining Mortality in non-Traumatic Subarachnoid Hemorrhage Joual: Clin Neurol Neurosurg. 2021 Aug;207:106813.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103644	E2094Hu	48T	$320		Add to Cart
BHE12103644	E2094Hu	96T	$458		Add to Cart

Human Glial Fibrillary Acidic Protein - GFAP ELISA Kit

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