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Home / ELISA Kit

Human Glia-derived Nexin - SERPINE2 ELISA Kit

SKU : BHE12106690

Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin.

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Bioassay Technology Laboratory

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Mfr.No.

E5327Hu

Sensitivity

0.052ng/ml

Detection Range

0.1-40ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

GDN;Glia-derived nexin;Peptidase inhibitor 7;PI7;PI-7;PN1;PN-1;PNI;Protease nexin 1;Protease nexin I;Serpin E2;SERPINE2

Molecular Weight

44002

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5327Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	19.61	0.63	3.21
Intra-Assay	2	18	6.1	0.25	4.13
Intra-Assay	3	18	13.37	0.64	4.76

Background

Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin.

Protocol

These standard curves of E5327Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	2.055	2.01	1.973
	1.966
12ng/ml	1.161	1.084	1.046
	1.007
6ng/ml	0.445	0.514	0.476
	0.583
3ng/ml	0.299	0.249	0.212
	0.199
1.5ng/ml	0.091	0.088	0.05
	0.084
0ng/ml	0.041	0.038	0
	0.034

Gene ID	5270
UniProt ID	P07093

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106690	E5327Hu	48T	$320		Add to Cart
BHE12106690	E5327Hu	96T	$458		Add to Cart

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