Human GFRA3 ELISA Kit PicoKine®

Mfr.No.

EK2075

Sensitivity

<20 pg/ml

Detection Range

46.9 pg/ml - 3,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	46.9	93.7	187.5	375	750	1500	3000
O.D.	0.036	0.075	0.144	0.245	0.468	0.821	1.315	1.908

Description

Human GFRA3 ELISA Kit PicoKine® (96 Tests). Quantitate Human GFRA3 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

GDNF family receptor alpha-3; GDNF receptor alpha-3; GDNFR-alpha-3; GFR-alpha-3; GFRA3; UNQ339; PRO538; PRO3664

Immunogen

Expression system for standard: NS0; Immunogen sequence: D32-N374

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human GFRA3

Subcellular Localization

Cell membrane. GPI-anchor.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	49	225	1375	62	303	1421
Standard deviation	2.50	11.93	75.63	4.09	20.60	102.31
CV (%)	5.1%	5.3%	5.5%	6.6%	6.8%	7.2%

Protein Function

Receptor for the glial cell line-derived neurotrophic factor, ARTN (artemin). Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase.

Background

GDNF family receptor alpha-3 (GFR?3), also known as the artemin receptor, is a protein that in humans is encoded by the GFRA3 gene. It is mapped to 5q31.2. The protein encoded by this gene is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor and a member of the GDNF receptor family. It forms a signaling receptor complex with RET tyrosine kinase receptor and binds the ligand, artemin.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	49	53	50	54	51	2.06	4.0%
Sample 2	225	222	233	208	222	9.02	4.0%
Sample 3	1375	1355	1340	1235	1326	54.12	4.0%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	2676
Gene Name	GFRA3
UniProt ID	O60609
Tissue Specificity	Widely expressed in adult and fetus which exhibit a similar patte. Essentially not expressed in the central nervous system, but highly expressed in several sensory and sympathetic ganglia of the peripheral nervous system. Moderate expression in many non-neuronal tissues, particularly those of the digestive and urogenital systems, but high expression in stomach and appendix. Several types of glandular tissues show low expression. Very low or no expression detected in the hematopoietic system.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001587	EK2075	96 wells/kit	$499		Add to Cart

Human GFRA3 ELISA Kit PicoKine®

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