Human General Transcription Factor Iif Subunit 1

Mfr.No.

E2710Hu

Sensitivity

7.11ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

General transcription factor IIF 74 kDa subunit;General transcription factor IIF subunit 1;GTF2F1;TFIIF-alpha;Transcription initiation factor IIF subunit alpha;Transcription initiation factor RAP74

Molecular Weight

58240

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2710Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	863.2	34	3.9
Intra-Assay	2	18	213	11.58	5.4
Intra-Assay	3	18	554.4	20.63	3.7

Background

TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.

Protocol

These standard curves of E2710Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.284	2.224	2.171
	2.163
800ng/L	1.343	1.35	1.297
	1.357
400ng/L	0.729	0.758	0.705
	0.786
200ng/L	0.417	0.486	0.433
	0.554
100ng/L	0.262	0.247	0.194
	0.232
0ng/L	0.056	0.053	0
	0.049

Gene ID	2962
UniProt ID	P35269

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104248	E2710Hu	48T	$320		Add to Cart
BHE12104248	E2710Hu	96T	$458		Add to Cart

Human General Transcription Factor Iif Subunit 1 - GTF2F1 ELISA Kit

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