Matrices listed below were spiked with certain level of GDNF and the recovery rates were calculated by comparing the measured value to the expected amount of GDNF in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-98
94
EDTA plasma(n=5)
88-100
94
heparin plasma(n=5)
87-95
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GDNF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
GDNF ELISA Kit allows for the in vitro quantitative determination of GDNF concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human GDNF(Glial cell line-derived neurotrophic factor) ELISA Kit