Matrices listed below were spiked with certain level of GDF15 and the recovery rates were calculated by comparing the measured value to the expected amount of GDF15 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-97
92
EDTA plasma(n=5)
88-102
97
heparin plasma(n=5)
91-105
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GDF15 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
GDF15 ELISA Kit allows for the in vitro quantitative determination of GDF15 concentrations in serum, plasma, tissue homogenates and other biological fluids.