Matrices listed below were spiked with certain level of GCH1 and the recovery rates were calculated by comparing the measured value to the expected amount of GCH1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-105
99
EDTA plasma(n=5)
91-100
96
heparin plasma(n=5)
90-104
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GCH1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-104%
88-103%
89-104%
EDTA plasma(n=5)
87-100%
83-99%
83-100%
heparin plasma(n=5)
80-96%
80-97%
86-100%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
P30793
Applications
ELISA
Applications Notes
GCH1 ELISA Kit allows for the in vitro quantitative determination of GCH1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human GCH1 (GTP cyclohydrolase 1) ELISA Kit