Matrices listed below were spiked with certain level of GBP2 and the recovery rates were calculated by comparing the measured value to the expected amount of GBP2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-103
96
EDTA plasma(n=5)
95-101
98
heparin plasma(n=5)
87-101
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GBP2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-103%
85-101%
85-101%
EDTA plasma(n=5)
82-97%
86-94%
83-97%
heparin plasma(n=5)
82-100%
80-92%
88-99%
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Notes
For Research Use Only
UniProt ID
P32456
Applications
ELISA
Applications Notes
GBP2 ELISA Kit allows for the in vitro quantitative determination of GBP2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Storage
2-8 °C for 6 months
Reviews of Human GBP2 (Guanylate-binding protein 2) ELISA Kit