Human Gastric Triacylglycerol Lipase

Mfr.No.

E2278Hu

Sensitivity

0.26ng/ml

Detection Range

0.5-150ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Gastric lipase;Gastric triacylglycerol lipase;GL;LIPF

Molecular Weight

45238

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2278Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	13	0.95	7.4
Intra-Assay	2	18	56.2	1.6	2.8
Intra-Assay	3	18	30.7	1.62	5.3

Background

Catalyzes the hydrolysis of triacylglycerols to yield free fatty acids, diacylglycerol, monoacylglycerol, and glycerol (PubMed: 2243091, PubMed: 10358049). Shows a preferential hydrolysis at the sn-3 position of triacylglycerol (PubMed: 2243091).

Protocol

These standard curves of E2278Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
80ng/ml	2.413	2.313	2.27
	2.213
40ng/ml	1.227	1.245	1.203
	1.264
20ng/ml	0.687	0.736	0.693
	0.785
10ng/ml	0.545	0.506	0.463
	0.467
5ng/ml	0.245	0.277	0.234
	0.309
0ng/ml	0.041	0.043	0
	0.044

Gene ID	8513
UniProt ID	P07098

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103818	E2278Hu	48T	$320		Add to Cart
BHE12103818	E2278Hu	96T	$458		Add to Cart

Human Gastric Triacylglycerol Lipase - LIPF ELISA Kit

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