Human gamma glutamyl transpeptidase,GGT ELISA Kit

Human gamma glutamyl transpeptidase,GGT ELISA Kit

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Mfr.No.

CSB-EL009394HU

Sensitivity

0.195 mU/mL

Detection Range

0.78 mU/mL-50 mU/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

gamma-glutamyltransferase 1, CD224, D22S672, D22S732, GGT, GTG, MGC96892, MGC96904, MGC96963, OTTHUMP00000028921, OTTHUMP00000159078, gamma-glutamyl transpeptidase, glutamyl transpeptidase

Recovery

The recovery of rat DHEA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-92
EDTA plasma (n=4)	94	88-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat DHEA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	102
	Range %	98-107
1:2	Average %	92
	Range %	88-96
1:4	Average %	89
	Range %	85-93
1:8	Average %	100
	Range %	97-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
10	0.162	0.158	0.160
2.5	0.307	0.298	0.303
0.8	0.487	0.504	0.496
0.2	0.801	0.810	0.806
0.05	1.224	1.305	1.265
0	2.000	1.899	1.950

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P19440

Applications	ELISA
Research Area	GGT1

Storage	Store at 2-8°C. Please refer to protocol.

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.