Human Galectin 7(Gal-7) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E13454h

Sensitivity

19.5 pg/mL

Detection Range

78 pg/mL-5000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Recovery

The recovery of mouse VEGF-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-93
EDTA plasma (n=4)	93	90-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse VEGF-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-102
1:2	Average %	91
	Range %	84-97
1:4	Average %	90
	Range %	87-94
1:8	Average %	100
	Range %	95-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P47929

Applications	ELISA
Research Area	Gal-7

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Identification of Differentially Expressed Proteins in the Serum of Colorectal Cancer Patients Using 2D-DIGE Proteomics Analysis Lay Cheng Lim,.et al,Pathology & Oncology Research,2016 22(1):169-177.

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