Human Galectin-4 ELISA Kit PicoKine®

Mfr.No.

EK1275

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.022	0.081	0.169	0.286	0.532	1.151	1.597	1.880

Description

Human Galectin-4 ELISA Kit PicoKine® (96 Tests). Quantitate Human LGALS4 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Galectin-4;Gal-4;Antigen NY-CO-27;L-36 lactose-binding protein;L36LBP;Lactose-binding lectin 4;LGALS4;

Molecular Weight

35941 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-I323

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Galectin-4

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	60	243	879	64	223	865
Standard deviation	3.24	19.19	45.7	4.28	19.4	47.57
CV (%)	5.4%	7.9%	5.2%	6.7%	8.7%	5.5%

Protein Function

Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions.

Background

Galectin-4, also known as LGALS4 or GAL4, is a protein that in humans is encoded by the LGALS4 gene. It is mapped to 19q13.2. Galectins are a family of animal lectins defined by an affinity for beta-galactoside-containing saccharides and by shared sequence elements. Galectin-4 is expressed as a 38-kD protein in the human colon adenocarcinoma T84 cell line. Galectin-4 is an S-type lectin that is strongly underexpressed in colorectal cancer, and this gene plays a role in cell adhesion. It has been found that decreased Galectin-4 expression occurs early in colorectal carcinogenesis.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	60	54	60	64	59	3.57	6%
Sample 2	243	258	258	260	254	6.83	2.6%
Sample 3	879	923	848	978	907	48.89	5.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3960
Gene Name	LGALS4
UniProt ID	P56470
Protein Name	Galectin-4
Sequence	Contains 2 galectin domains.

Applications	ELISA
Research Area	Cancer,Cell Adhesion,2339,Cytoskeleton/ECM,Energy Metabolism,Energy Transfer Pathways,Epigenetics and Nuclear Signaling,Metabolic Signaling Pathways,Metabolism,Pathways and Processes,Signal Transduction,Tags & Cell Markers,Transcription,Transcription Factors

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000865	EK1275	96 wells/kit	$750		Add to Cart

Human Galectin-4 ELISA Kit PicoKine®

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