Human Galectin-3BP ELISA Kit PicoKine®

Mfr.No.

EK1240

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.022	0.094	0.144	0.278	0.520	0.902	1.468	2.070

Description

Human Galectin-3BP ELISA Kit PicoKine® (96 Tests). Quantitate Human LGALS3BP in cell culture supeatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Galectin-3-binding protein;Basement membrane autoantigen p105;Lectin galactoside-binding soluble 3-binding protein;Mac-2-binding protein;MAC2BP;Mac-2 BP;Tumor-associated antigen 90K;LGALS3BP;M2BP;

Molecular Weight

65331 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: V19-D585

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Galectin-3BP

Subcellular Localization

Secreted . Secreted, extracellular space, extracellular matrix .

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	312	1090	4158	326	1154	3768
Standard deviation	18.72	63.22	282.74	20.86	84.24	271.29
CV (%)	6%	5.8%	6.8%	6.4%	7.3%	7.2%

Protein Function

Promotes intergrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells. .

Background

Galectin-3-binding protein is a protein that in humans is encoded by the LGALS3BP gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cellcell and cellmatrix interactions. Using fluorescence insitu hybridization, the full length 90K cDNA has been localized to chromosome 17q25. The native protein binds specifically to a human macrophage-associated lectin known as Mac-2 and also binds to galectin 1.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	312	367	361	366	351	22.91	6.5%
Sample 2	1090	1067	1143	1041	1085	37.57	3.4%
Sample 3	4158	3836	4350	4159	4125	184.65	4.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3959
Gene Name	LGALS3BP
UniProt ID	Q08380
Protein Name	Galectin-3-binding protein
Tissue Specificity	Ubiquitous. Detected in body fluids such as semen, milk, serum, tears, saliva and urine. Expressed by keratinocytes and fibroblasts. .

Applications	ELISA
Research Area	Cancer,Cell Cycle,Cell Division,Oncoproteins,Tumor Biomarkers

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001018	EK1240	96 wells/kit	$399		Add to Cart

Human Galectin-3BP ELISA Kit PicoKine®

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