Human Galectin-3-binding protein(LGALS3BP) ELISA kit

Human Galectin-3-binding protein(LGALS3BP) ELISA kit

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Mfr.No.

CSB-EL012888HU

Sensitivity

0.39 ng/mL

Detection Range

1.56 ng/mL-100 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

lectin, galactoside-binding, soluble, 3 binding protein, 90K, BTBD17B, MAC-2-BP, L3 antigen, Mac-2-binding protein, galectin 3 binding protein, serum protein 90K

Recovery

The recovery of human ITIH1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-98
EDTA plasma (n=4)	97	90-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ITIH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	86
	Range %	80-92
1:200	Average %	97
	Range %	91-105
1:400	Average %	99
	Range %	92-110
1:800	Average %	97
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.400	2.418	2.409	2.328
500	2.088	1.999	2.004	1.923
250	1.562	1.555	1.559	1.478
125	1.228	1.211	1.220	1.139
62.5	0.689	0.665	0.677	0.596
31.25	0.452	0.436	0.444	0.363
15.6	0.270	0.254	0.262	0.181
0	0.084	0.078	0.081	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q08380

Applications	ELISA
Research Area	LGALS3BP

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502459	CSB-EL012888HU	96T	$695	Add to Cart
BHE10502459	CSB-EL012888HU	5x96T	$3336	Add to Cart
BHE10502459	CSB-EL012888HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.