Human Galectin-2 ELISA Kit PicoKine®

Mfr.No.

EK1278

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5,000	10,000
O.D.	0.028	0.098	0.143	0.258	0.526	0.936	1.525	2.002

Description

Human Galectin-2 ELISA Kit PicoKine® (96 Tests). Quantitate Human LGALS2 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Galectin-2;Gal-2;Beta-galactoside-binding lectin L-14-II;HL14;Lactose-binding lectin 2;S-Lac lectin 2;LGALS2;

Molecular Weight

14644 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: M1-E132

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Galectin-2

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	288	1975	5128	263	1848	5204
Standard deviation	21.31	100.72	302.55	23.4	129.36	369.48
CV (%)	7.4%	7.1%	5.9%	8.9%	7%	7.1%

Protein Function

This protein binds beta-galactoside. Its physiological function is not yet known.

Background

Galectin-2 is a protein that in humans is encoded by the LGALS2 gene. It is mapped to 22q13.1. The protein encoded by this gene is a soluble beta-galactoside binding lectin. The encoded protein is found as a homodimer and can bind to lymphotoxin-alpha. It has been found that a single-nucleotide polymorphism in Galectin-2 is significantly associated with susceptibility to myocardial infarction. This genetic substitution affects the transcriptional level of Galectin-2 in vitro, potentially leading to altered secretion of LTA, to which Galectin-2 binds, which would then affect the degree of inflammation.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	288	323	331	326	317	16.98	5.3%
Sample 2	1975	1974	1898	1989	1959	35.71	1.8%
Sample 3	5128	5263	5282	4554	5056	296.27	5.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3957
Gene Name	LGALS2
UniProt ID	P05162
Protein Name	Galectin-2
Sequence	Contains 1 galectin domain.

Applications	ELISA
Research Area	Cell Adhesion Proteins,Cell Biology,Cell Cycle,Cell Differentiation,Neuroscience

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000868	EK1278	96 wells/kit	$750		Add to Cart

Human Galectin-2 ELISA Kit PicoKine®

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