Human Galectin-1(LGALS1) ELISA kit

Human Galectin-1(LGALS1) ELISA kit

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Mfr.No.

CSB-EL012882HU

Sensitivity

0.78 ng/mL

Detection Range

3.12 ng/mL-200 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

lectin, galactoside-binding, soluble, 1, DKFZp686E23103, GAL1, GBP, S-Lac lectin 1, beta-galactoside-binding lectin L-14-I, galaptin, galectin 1, galectin-1, lactose-binding lectin 1

Recovery

The recovery of pig leptin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-102
EDTA plasma (n=4)	92	88-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig leptin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-102
1:2	Average %	92
	Range %	87-98
1:4	Average %	95
	Range %	91-100
1:8	Average %	93
	Range %	84-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
0	2.665	2.529	2.597
0.25	1.461	1.387	1.424
1	0.973	1.008	0.991
4	0.500	0.513	0.507
12.5	0.346	0.334	0.340
50	0.150	0.155	0.153

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P09382

Applications	ELISA
Research Area	LGALS1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502457	CSB-EL012882HU	96T	$695	Add to Cart
BHE10502457	CSB-EL012882HU	5x96T	$3336	Add to Cart
BHE10502457	CSB-EL012882HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.