Human Galactomannan - GM ELISA Kit

Mfr.No.

E3948Hu

Description

This sandwich kit is for the accurate quantitative detection of human Galactomannan (also known as GM) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Galactokinase;Galactose kinase;GALK;GK1;GM;HEL-S-19

Reactivity

Human

Detection Range

0.2-60ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

42272

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.08ng/ml

Background

Galactomannans are polysaccharides consisting of a mannose backbone with galactose side groups (more specifically, a (1-4)-linked beta-D-mannopyranose backbone with branchpoints from their 6-positions linked to alpha-D-galactose, (i.e. 1-6-linked alpha-D-galactopyranose).

Gene ID	2584
UniProt ID	P51570
Antigen	GM

Research Area

Immunology

Protocol

These standard curves of E3948Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40ng/ml	2.018	2.183	2.128
	2.348
20ng/ml	1.404	1.385	1.33
	1.366
10ng/ml	0.806	0.823	0.768
	0.841
5ng/ml	0.529	0.47	0.415
	0.411
2.5ng/ml	0.22	0.262	0.207
	0.304
0ng/ml	0.06	0.055	0
	0.05

Precision

We measured random samples of E3948Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	26.75	0.91	3.39
Intra-Assay	2	18	16.4	1.02	6.2
Intra-Assay	3	18	7.47	0.49	6.54

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105382	E3948Hu	48T	$320		Add to Cart
BHE12105382	E3948Hu	96T	$458		Add to Cart

Human Galactomannan - GM ELISA Kit

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