Human G1 S-specific Cyclin-E1

Mfr.No.

E5313Hu

Alternative Names

CCNE;CCNE1;G1/S-specific cyclin-E1;pCCNE1

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

47077

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.56ng/L

Background

G1/S-specific cyclin-E1 is a protein that in humans is encoded by the CCNE1 gene. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event.

Gene ID	898
UniProt ID	P24864
Antigen	CCNE1

Research Area

Cell Biology

Protocol

These standard curves of E5313Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.195	2.149	2.091
	2.102
800ng/L	1.452	1.365	1.306
	1.277
400ng/L	0.811	0.831	0.772
	0.85
200ng/L	0.525	0.518	0.46
	0.511
100ng/L	0.272	0.263	0.205
	0.254
0ng/L	0.075	0.058	0
	0.042

Precision

We measured random samples of E5313Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	566.16	35.52	6.27
Intra-Assay	2	18	1000.23	36.77	3.68
Intra-Assay	3	18	317.84	23.92	7.53

Storage	2-8°C

Reference	Copy number variations and protein expression of Cyclin E1 and Epithelial cell transforming sequence 2 genes predict the chemotherapeutic response in patients with serous ovarian carcinoma Journal: Pak J Med Sci. 2023 May-Jun;39(3):835-842.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106676	E5313Hu	48T	$320		Add to Cart
BHE12106676	E5313Hu	96T	$458		Add to Cart

Human G1 S-specific Cyclin-E1 - CCNE1 ELISA Kit

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