Human G Protein Coupled Receptor 84

Mfr.No.

E6720Hu

Sensitivity

9.78ng/L

Detection Range

20-4500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

GPR 84;GPR84;G-protein coupled receptor 84;Inflammation-related G-protein coupled receptor EX33

Molecular Weight

43705

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E6720Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	207.6	9.8	4.7
Intra-Assay	2	18	1147.3	83.44	7.3
Intra-Assay	3	18	330.1	17.08	5.2

Background

Receptor for medium-chain free fatty acid (FFA) with carbon chain lengths of C9 to C14. Capric acid (C10:0), undecanoic acid (C11:0) and lauric acid (C12:0) are the most potent agonists. Not activated by short-chain and long-chain saturated and unsaturated FFAs. Activation by medium-chain free fatty acid is coupled to a pertussis toxin sensitive G(i/o) protein pathway. May have important roles in processes from fatty acid metabolism to regulation of the immune system.

Protocol

These standard curves of E6720Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.05	2.095	2.059
	2.141
1200ng/L	1.836	1.774	1.738
	1.713
600ng/L	1.31	1.271	1.235
	1.232
300ng/L	0.745	0.71	0.674
	0.674
150ng/L	0.361	0.368	0.332
	0.375
0ng/L	0.03	0.036	0
	0.042

Gene ID	53831
UniProt ID	Q9NQS5

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115610	E6720Hu	48T	$320		Add to Cart
BHE12115610	E6720Hu	96T	$458		Add to Cart

Human G Protein Coupled Receptor 84 - GPR84 ELISA Kit

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