Human G protein-coupled bile acid receptor 1(GPBAR1) ELISA Kit

Human G protein-coupled bile acid receptor 1(GPBAR1) ELISA Kit

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Mfr.No.

CSB-E13937h

Alternative Names

G protein-coupled bile acid receptor 1, BG37, GPCR, GPCR19, GPR131, M-BAR, MGC40597, TGR5, G-protein coupled bile acid receptor BG37, membrane bile acid receptor, membrane-type receptor for bile acids

Reactivity

Human

Detection Range

0.156 ng/mL-10 ng/mL

Sample Type

Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human DLL3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	96	91-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human DLL3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	105
	Range %	100-112
1:10	Average %	97
	Range %	91-105
1:20	Average %	93
	Range %	89-100
1:40	Average %	95
	Range %	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.199	2.354	2.277	2.134
600	1.714	1.811	1.763	1.620
300	1.172	1.104	1.138	0.995
150	0.698	0.674	0.686	0.543
75	0.425	0.407	0.416	0.273
37.5	0.310	0.329	0.320	0.177
18.75	0.207	0.219	0.213	0.070
0	0.141	0.145	0.143

Species

Homo sapiens

Sensitivity

0.039 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q8TDU6
Antigen	G protein-coupled bile acid receptor 1

Applications

ELISA

Research Area

GPBAR1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502574	CSB-E13937h	96T	$790	Add to Cart
BHE10502574	CSB-E13937h	5x96T	$3792	Add to Cart
BHE10502574	CSB-E13937h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.