Matrices listed below were spiked with certain level of FXIIa and the recovery rates were calculated by comparing the measured value to the expected amount of FXIIa in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
96
EDTA plasma(n=5)
88-104
95
heparin plasma(n=5)
93-105
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FXIIa and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
FXIIa ELISA Kit allows for the in vitro quantitative determination of FXIIa concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human FXIIa(Activated Coagulation Factor XII) ELISA Kit