Matrices listed below were spiked with certain level of FUS and the recovery rates were calculated by comparing the measured value to the expected amount of FUS in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-98
92
EDTA plasma(n=5)
88-98
93
heparin plasma(n=5)
90-105
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FUS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-105%
87-104%
92-99%
EDTA plasma(n=5)
89-99%
82-100%
83-98%
heparin plasma(n=5)
80-95%
81-95%
81-97%
Sensitivity
< 46.875pg/ml
Background
75 kDa DNA-pairing protein/Oncogene FUS/Oncogene TLS/POMp75/Translocated in liposarcoma protein/TLS
Notes
For Research Use Only
UniProt ID
P35637
Antigen
FUS
Applications
ELISA
Applications Notes
FUS ELISA Kit allows for the in vitro quantitative determination of FUS concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human FUS(RNA-binding protein FUS) ELISA Kit