Human Furin ELISA Kit PicoKine®

Mfr.No.

EK0916

Sensitivity

<10 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	312	625	200000000	20000000	2000000	200000	20000
O.D.	0.015	0.055	0.096	0.177	0.346	0.704	1.343	1.882

Description

Human Furin ELISA Kit PicoKine® (96 Tests). Quantitate Human FURIN in cell culture supeatants and cell lysates. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Furin;3.4.21.75;Dibasic-processing enzyme;Paired basic amino acid residue-cleaving enzyme;PACE;FURIN;FUR, PACE, PCSK3;

Molecular Weight

86678 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: D108-E715

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Furin

Subcellular Localization

Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein. Cell membrane; Single-pass type I membrane protein. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin.

Sample Type

Cell culture supeatants, Cell lysates

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	417	2751	82701	457	2701	7870
Standard deviation	21.68	143.05	464.4	28.79	148.55	448.59
CV (%)	5.2%	5.2%	5.6%	5.5%%	5.5%	5.7%

Protein Function

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R)R consensus motif. .

Background

Furin (Fur), also known as PACE, is a protein that in humans is encoded by the FURIN gene. The protein encoded by this gene is an enzyme which belongs to the subtilisin-like proprotein convertase family. The FUR gene is located approximately 1 kb upstream of the FES gene on chromosome 15q25-q26. This gene is thought to play a role in tumor progression. Furin is one of the proteases responsible for the proteolytic cleavage of HIV envelope polyprotein precursor gp160 to gp120 and gp41 prior to viral assembly. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. Expression of furin in T-cells is required for maintenance of peripheral immune tolerance

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	417	420	397	384	404	14.77	3.6%
Sample 2	2751	2551	2738	2985	2756	153.96	5.5%
Sample 3	8293	8993	7686	9246	8554	610.96	7.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	5045
Gene Name	FURIN
UniProt ID	P09958
Protein Name	Furin
Tissue Specificity	Seems to be expressed ubiquitously.
Sequence	Belongs to the peptidase S8 family. Furin subfamily.

Applications	ELISA
Research Area	Amino Acids,Cell Adhesion,Cell Biology,Cytoskeleton/ECM,Integrins,Metabolism,Proteolysis/Ubiquitin,Proteolytic Enzymes,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21000693	EK0916	96 wells/kit	$499		Add to Cart

Human Furin ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human Furin ELISA Kit PicoKine®

Rate it now..!!