Human FUNDC1(FUN14 domain-containing protein 1) ELISA Kit

Mfr.No.

ELK9459

Alternative Names

FUNDC1, FUN14 domain-containing protein 1, FUNDC1; FUN14 domain-containing protein 1;

Reactivity

Human

Detection Range

78.13-5000 pg/mL, 78.13-5000 pg/mL

Sample Type

Other biological fluids, Serum, Plasma

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant FUNDC1 and the recovery rates were calculated by comparing the measured value to the expected amount of FUNDC1 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-92%	86%
EDTA plasma(n=5)	84-92%	87%
Heparin plasma(n=5)	78-95%	86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FUNDC1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	79-92%	86-97%	88-95%	92-101%
EDTA plasma(n=5)	96-105%	92-103%	82-96%	87-93%
Heparin plasma(n=5)	95-106%	79-91%	89-103%	82-95%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
5000.00	1.972	1.877
2500.00	1.638	1.543
1250.00	1.088	0.993
625.00	0.861	0.766
312.50	0.556	0.461
156.25	0.377	0.282
78.13	0.235	0.140
0.00	0.095	0.000

Sensitivity

31 pg/mL

Limit Of Detection

5000 pg/mL

UniProt ID	Q8IVP5
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FUNDC1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FUNDC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FUNDC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FUNDC1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Cell Biology
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE152011197	ELK9459	48T	$320		Add to Cart
BHE152011197	ELK9459	96T	$458		Add to Cart

Human FUNDC1(FUN14 domain-containing protein 1) ELISA Kit

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