Human FTO(Alpha-ketoglutaRate-dependent dioxygenase FTO) ELISA Kit

Mfr.No.

ELK9481

Alternative Names

FTO, KIAA1752, fat mass and obesity associated, FTO; KIAA1752; fat mass and obesity associated

Reactivity

Human

Detection Range

0.16-10 ng/mL, 0.16-10 ng/mL

Sample Type

Other biological fluids, Serum, Plasma

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant FTO and the recovery rates were calculated by comparing the measured value to the expected amount of FTO in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	93%
EDTA plasma(n=5)	78-96%	87%
Heparin plasma(n=5)	85-99%	92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FTO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-97%	90-99%	82-101%	85-94%
EDTA plasma(n=5)	79-92%	83-95%	86-97%	79-92%
Heparin plasma(n=5)	89-102%	88-101%	90-98%	92-106%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
10.00	1.929	1.841
5.00	1.607	1.519
2.50	1.227	1.139
1.25	0.796	0.708
0.63	0.579	0.491
0.32	0.389	0.301
0.16	0.226	0.138
0.00	0.088	0.000

Sensitivity

0.059 ng/mL

Limit Of Detection

10 ng/mL

UniProt ID	Q9C0B1
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FTO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FTO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FTO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FTO in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Enzyme & Kinase
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE152011219	ELK9481	48T	$320		Add to Cart
BHE152011219	ELK9481	96T	$458		Add to Cart

Human FTO(Alpha-ketoglutaRate-dependent dioxygenase FTO) ELISA Kit

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