Human Fructosamine-3-kinase

Mfr.No.

E3233Hu

Sensitivity

0.054ng/ml

Detection Range

0.1-40ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

FN 3K;FN3K;Fructosamine-3-kinase;Protein-psicosamine 3-kinase FN3K;Protein-ribulosamine 3-kinase FN3K

Molecular Weight

35171

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E3233Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	15.4	0.48	3.1
Intra-Assay	2	18	11.6	0.42	3.7
Intra-Assay	3	18	4.5	0.22	4.8

Background

Fructosamine-3-kinase involved in protein deglycation by mediating phosphorylation of fructoselysine residues on glycated proteins, to generate fructoselysine-3 phosphate (PubMed: 11016445, PubMed: 11522682, PubMed: 11975663). Fructoselysine-3 phosphate adducts are unstable and decompose under physiological conditions (PubMed: 11522682, PubMed: 11975663). Involved in intracellular deglycation in erythrocytes (PubMed: 11975663). Involved in the response to oxidative stress by mediating deglycation of NFE2L2/NRF2, glycation impairing NFE2L2/NRF2 function (By similarity). Also able to phosphorylate psicosamines and ribulosamines (PubMed: 14633848).

Protocol

These standard curves of E3233Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	1.938	1.888	1.848
	1.839
12ng/ml	1.053	0.987	0.947
	0.922
6ng/ml	0.524	0.5	0.459
	0.476
3ng/ml	0.22	0.253	0.212
	0.285
1.5ng/ml	0.143	0.138	0.097
	0.133
0ng/ml	0.035	0.041	0
	0.047

Gene ID	64122
UniProt ID	Q9H479

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104762	E3233Hu	48T	$320		Add to Cart
BHE12104762	E3233Hu	96T	$458		Add to Cart

Human Fructosamine-3-kinase - FN3K ELISA Kit

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