Human Frizzled-5 - FZD5 ELISA Kit

Mfr.No.

E2362Hu

Sensitivity

0.047ng/ml

Detection Range

0.1-35ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

C2orf31;Frizzled-5;Fz-5;FZD5;FzE5;HFZ5

Molecular Weight

64507

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2362Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2.58	0.12	4.63
Intra-Assay	2	18	7.64	0.48	6.28
Intra-Assay	3	18	2.5	0.15	5.88

Background

Frizzled-5 is a protein that in humans is encoded by the FZD5 gene. Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD5 protein is believed to be the receptor for the Wnt5A ligand.

Protocol

These standard curves of E2362Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
20ng/ml	2.288	2.202	2.164
	2.116
10ng/ml	1.927	1.895	1.857
	1.863
5ng/ml	1.184	1.236	1.198
	1.288
2.5ng/ml	0.717	0.689	0.65
	0.66
1.25ng/ml	0.429	0.408	0.37
	0.388
0ng/ml	0.028	0.038	0
	0.049

Gene ID	7855
UniProt ID	Q13467

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103900	E2362Hu	48T	$320		Add to Cart
BHE12103900	E2362Hu	96T	$458		Add to Cart

Human Frizzled-5 - FZD5 ELISA Kit

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