Human free haemoglobin,f-Hb ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E09632h

Sensitivity

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Detection Range

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Reactivity

Human

Assay Duration

1-5h

Alternative Names

free haemoglobin,f-Hb

Recovery

The recovery of human C1QA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	86-97
EDTA plasma (n=4)	93	86-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human C1QA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	97
	Range %	90-101
1:200	Average %	103
	Range %	98-107
1:400	Average %	95
	Range %	89-99
1:800	Average %	97
	Range %	93-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	f-Hb

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502440	CSB-E09632h	96T	$790	Add to Cart
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BHE10502440	CSB-E09632h	10x96T	$7505	Add to Cart