Human fragile histidine triad (FHIT) ELISA Kit

Human fragile histidine triad (FHIT) ELISA Kit

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Mfr.No.

CSB-E14182h

Sensitivity

7.8 pg/mL

Detection Range

31.2 pg/mL-2000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

fragile histidine triad gene, AP3Aase, FRA3B, AP3A hydrolase, bis(5 -adenosyl)-triphosphatase, diadenosine 5 ,5 -P1,P3-triphosphate hydrolase, dinucleosidetriphosphatase, tumor suppressor protein

Recovery

The recovery of human Cav-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	95-107
EDTA plasma (n=4)	95	89-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human Cav-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-98
1:2	Average %	97
	Range %	92-103
1:4	Average %	89
	Range %	85-96
1:8	Average %	93
	Range %	87-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.501	2.552	2.527	2.424
1000	1.863	2.002	1.933	1.830
500	1.254	1.289	1.272	1.169
250	0.821	0.833	0.827	0.724
125	0.498	0.535	0.517	0.414
62.5	0.328	0.346	0.337	0.234
31.25	0.206	0.201	0.204	0.101
0	0.104	0.101	0.103	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P49789

Applications	ELISA
Research Area	FHIT

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502436	CSB-E14182h	96T	$790	Add to Cart
BHE10502436	CSB-E14182h	5x96T	$3792	Add to Cart
BHE10502436	CSB-E14182h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.