Matrices listed below were spiked with certain level of FOXI2 and the recovery rates were calculated by comparing the measured value to the expected amount of FOXI2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
81-101
93
EDTA plasma(n=5)
80-105
90
heparin plasma(n=5)
93-97
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FOXI2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
80-103%
83-105%
80-103%
EDTA plasma(n=5)
90-99%
90-98%
95-100%
heparin plasma(n=5)
92-99%
93-100%
93-100%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
Q6ZQN5
Applications
ELISA
Applications Notes
FOXI2 ELISA Kit allows for the in vitro quantitative determination of FOXI2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human FOXI2 (Forkhead box protein I2)ELISA Kit