Human Formin-binding Protein 1-like

Mfr.No.

E3189Hu

Sensitivity

7.98ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

FNBP 1L;FNBP1L;Formin-binding protein 1-like;Toca-1;Transducer of Cdc42-dependent actin assembly protein 1

Molecular Weight

70065

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E3189Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	210.6	12.58	6
Intra-Assay	2	18	633.6	35.24	5.6
Intra-Assay	3	18	861.3	36.56	4.2

Background

Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. May bind to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promote membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by activating the WASL/N-WASP-WASPIP/WIP complex, the predominant form of WASL/N-WASP in cells. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Essential for autophagy of intracellular bacterial pathogens.

Protocol

These standard curves of E3189Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.343	2.248	2.194
	2.154
800ng/L	1.467	1.374	1.32
	1.281
400ng/L	0.769	0.802	0.748
	0.835
200ng/L	0.549	0.501	0.447
	0.453
100ng/L	0.275	0.27	0.216
	0.266
0ng/L	0.051	0.054	0
	0.057

Gene ID	54874
UniProt ID	Q5T0N5

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104718	E3189Hu	48T	$320		Add to Cart
BHE12104718	E3189Hu	96T	$458		Add to Cart

Human Formin-binding Protein 1-like - FNBP1L ELISA Kit

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