Human forkhead box O3,FOXO3 ELISA Kit

Human forkhead box O3,FOXO3 ELISA Kit

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Mfr.No.

CSB-E11177h

Sensitivity

0.39 pg/mL

Detection Range

1.56 pg/mL-100 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

forkhead box O3, AF6q21, DKFZp781A0677, FKHRL1, FKHRL1P2, FOXO2, FOXO3A, MGC12739, MGC31925, forkhead box O3A, forkhead homolog (rhabdomyosarcoma) like 1, forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1

Recovery

The recovery of human MMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	80-98
EDTA plasma (n=4)	94	90-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-92
1:2	Average %	99
	Range %	91-107
1:4	Average %	96
	Range %	92-101
1:8	Average %	93
	Range %	86-112

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
50	2.659	2.588	2.624	2.435
25	2.210	2.080	2.145	1.956
12.5	1.540	1.580	1.560	1.371
6.25	1.021	1.009	1.015	0.826
3.12	0.588	0.620	0.604	0.415
1.56	0.390	0.420	0.405	0.216
0.78	0.250	0.260	0.255	0.066
0	0.190	0.188	0.189	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O43524

Applications	ELISA
Research Area	FOXO3

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502430	CSB-E11177h	96T	$695	Add to Cart
BHE10502430	CSB-E11177h	5x96T	$3336	Add to Cart
BHE10502430	CSB-E11177h	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.