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Home / ELISA Kit

Human follicle-stimulating hormone,FSH ELISA Kit

SKU : BHE10502420

This Rabbit IL12/P70 ELISA Kit was designed for the quantitative measurement of Rabbit IL12/P70 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.

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CUSABIO TECHONOLOGY LLC

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Mfr.No.

CSB-E06867h

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Recovery

The recovery of rabbit IL-12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type		Average % Recovery		Range
Serum (n=5)		95		86-110
EDTA plasma (n=4)		97		89-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		93
		Range %		89-95
1:2		Average %		90
		Range %		83-99
1:4		Average %		95
		Range %		92-102
1:8		Average %		93
		Range %		86-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.096	2.108	2.102	1.938
100	1.678	1.651	1.665	1.501
50	1.254	1.237	1.246	1.082
25	0.896	0.905	0.901	0.737
12.5	0.614	0.642	0.628	0.464
6.25	0.378	0.364	0.371	0.207
3.12	0.252	0.249	0.251	0.087
0	0.161	0.166	0.164

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Protocol

UniProt ID	P01225

Applications	ELISA
Research Area	FSH

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10502420	CSB-E06867h	96T	$395		Add to Cart
BHE10502420	CSB-E06867h	5x96T	$1896		Add to Cart
BHE10502420	CSB-E06867h	10x96T	$3752.50		Add to Cart

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