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Home / ELISA Kit

Human Focal adhesion kinase (FAK) ELISA Kit

SKU : BHE13703278

PTK2 is a focal adhesion-associated protein kinase involved in cellular adhesion and spreading processes. It has been shown that when FAK was blocked, breast cancer cells became less metastastic due to decreased mobility.FAK is a 125 Kd protein that is recruited as a participant in focal adhesion dynamics between cells and has a role in motility and cell survival. FAK is a highly conserved, non-receptor tyrosine kinase originally identified as a substrate for the oncogene protein tyrosine kinase v-src. This cytosolic kinase has been implicated in diverse cellular roles including cell locomotion, mitogen response and cell survival. FAK is typically located at structures known as focal adhesions, these are multi-protein structures that link the extracellular matrix (ECM) to the cytoplasmic cytoskeleton.

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Mfr.No.	KTE61062
Description	This Human Focal adhesion kinase (FAK) ELISA Kit employs a two-site sandwich ELISA to quantitate PTK2.
Alternative Names	PTK2; FADK; FAK; FAK1; FRNK; pp125FAK; FAK-related non-kinase polypeptide; focal adhesion kinase 1
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Focal adhesion kinase microplate Human Focal adhesion kinase standard Human Focal adhesion kinase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Focal adhesion kinase (FAK) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTK2. No significant cross-reactivity or interference between Human PTK2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	PTK2 is a focal adhesion-associated protein kinase involved in cellular adhesion and spreading processes. It has been shown that when FAK was blocked, breast cancer cells became less metastastic due to decreased mobility.FAK is a 125 Kd protein that is recruited as a participant in focal adhesion dynamics between cells and has a role in motility and cell survival. FAK is a highly conserved, non-receptor tyrosine kinase originally identified as a substrate for the oncogene protein tyrosine kinase v-src. This cytosolic kinase has been implicated in diverse cellular roles including cell locomotion, mitogen response and cell survival. FAK is typically located at structures known as focal adhesions, these are multi-protein structures that link the extracellular matrix (ECM) to the cytoplasmic cytoskeleton.
Notes	This Human Focal adhesion kinase (FAK) ELISA Kit employs a two-site sandwich ELISA to quantitate PTK2 in samples. An antibody specific for PTK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	5747
Antigen	PTK2
Tag	PTK2
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Focal adhesion kinase (FAK) ELISA Kit employs a two-site sandwich ELISA to quantitate PTK2 in samples. An antibody specific for PTK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703278	KTE61062	48 T	$359		Add to Cart
BHE13703278	KTE61062	96 T	$619		Add to Cart
BHE13703278	KTE61062	96 Tx5	$2809		Add to Cart
BHE13703278	KTE61062	96 Tx50	$26209		Add to Cart

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