Human FMS-like tyrosine kinase 3,Flt3 ELISA Kit

Human FMS-like tyrosine kinase 3,Flt3 ELISA Kit

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Mfr.No.

CSB-E04556h

Sensitivity

15.6 pg/mL

Detection Range

62.5 pg/mL-4000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

fms-related tyrosine kinase 3, RP11-153M24.3, CD135, FLK2, STK1, CD135 antigen, FL cytokine receptor, FLT3 receptor tyrosine kinase, fetal liver kinase 2, growth factor receptor tyrosine kinase type III, stem cell tyrosine kinase 1, ty

Recovery

The recovery of mouse Ox-HDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	90-97
EDTA plasma (n=4)	99	95-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Ox-HDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	95
	Range %	91-99
1:200	Average %	94
	Range %	90-98
1:400	Average %	98
	Range %	94-104
1:800	Average %	84
	Range %	80-88

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
1000	0.097	0.096	0.097
500	0.267	0.263	0.265
250	0.466	0.454	0.460
125	0.691	0.687	0.689
62.5	1.080	1.091	1.086
31.25	1.391	1.412	1.402
15.6	1.504	1.606	1.555
0	1.813	1.711	1.762

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P36888

Applications	ELISA
Research Area	FLT3

Storage	Store at 2-8°C. Please refer to protocol.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.