Human Flotillin-1(FLOT1) ELISA kit

Mfr.No.

CSB-EL008727HU

Sensitivity

3.12 pg/mL

Reactivity

Human

Sample Type

Cell lysates, Serum, Tissue homogenates, Plasma

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

flotillin 1, DADB-118P11.2, integral membrane component of caveolae

Recovery

The recovery of rat MMP-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	88-100
EDTA plasma (n=4)	95	89-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	91
	Range %	85-99
1:2	Average %	92
	Range %	90-103
1:4	Average %	95
	Range %	87-105
1:8	Average %	95
	Range %	87-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	O75955
Antigen	Flotillin 1

Applications	ELISA
Research Area	FLOT1

Storage	Store at 2-8°C. Please refer to protocol.

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