Matrices listed below were spiked with certain level of FLNalpha and the recovery rates were calculated by comparing the measured value to the expected amount of FLNalpha in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-101
96
EDTA plasma(n=5)
86-98
92
heparin plasma(n=5)
95-105
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FLNalpha and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
FLNalpha ELISA Kit allows for the in vitro quantitative determination of FLNalpha concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human FLNalpha (Filamin A, Alpha) ELISA Kit