Human Filaggrin - FLG ELISA Kit

Mfr.No.

E2699Hu

Sensitivity

7.33ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Filaggrin (also known as FLG) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Filaggrin;FLG

Molecular Weight

435170

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2699Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	721.9	31.92	4.4
Intra-Assay	2	18	191.3	11.79	6.2
Intra-Assay	3	18	525.6	34.04	6.5

Background

Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.

Protocol

These standard curves of E2699Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.314	2.306	2.251
	2.298
800ng/L	1.273	1.329	1.274
	1.385
400ng/L	0.892	0.86	0.804
	0.827
200ng/L	0.456	0.49	0.434
	0.523
100ng/L	0.327	0.319	0.264
	0.312
0ng/L	0.043	0.055	0
	0.068

Gene ID	2312
UniProt ID	P20930

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104237	E2699Hu	48T	$320		Add to Cart
BHE12104237	E2699Hu	96T	$458		Add to Cart

Human Filaggrin - FLG ELISA Kit

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