Human Ficolin 3 - FCN3 ELISA Kit

Mfr.No.

E0329Hu

Description

This sandwich kit is for the accurate quantitative detection of Human Ficolin 3 (also known as FCN3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Collagen/fibrinogen domain-containing lectin 3 p35;Collagen/fibrinogen domain-containing protein 3;FCN 3;FCN3;Ficolin-3;Hakata antigen

Reactivity

Human

Detection Range

2-600ng/ml

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

32903

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

1.02ng/ml

Background

May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc-binding lectin. Has affinity with GalNAc, GlcNAc, D-fucose, as mono/oligosaccharide and lipopolysaccharides from S.typhimurium and S.minnesota.

Gene ID	8547
UniProt ID	O75636
Antigen	FCN3

Research Area

Immunology

Protocol

These standard curves of E0329Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
320ng/ml	2.349	2.259	2.211
	2.169
160ng/ml	1.333	1.31	1.262
	1.288
80ng/ml	0.806	0.754	0.706
	0.703
40ng/ml	0.473	0.487	0.438
	0.501
20ng/ml	0.316	0.322	0.274
	0.328
0ng/ml	0.05	0.048	0
	0.046

Precision

We measured random samples of E0329Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	120.1	5.94	4.9
Intra-Assay	2	18	252.1	5.27	2.1
Intra-Assay	3	18	32.4	2.33	7.2

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102034	E0329Hu	96T	$458		Add to Cart
BHE12102034	E0329Hu	48T	$320		Add to Cart

Human Ficolin 3 - FCN3 ELISA Kit

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