Human Ficolin-2(FCN2) ELISA kit

Human Ficolin-2(FCN2) ELISA kit

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Mfr.No.

CSB-EL008551HU

Sensitivity

0.06 ng/mL

Detection Range

0.25 ng/mL-16 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin), RP11-263F14.2, EBP-37, FCNL, P35, ficolin-2, L-ficolin, collagen/fibrinogen domain-containing protein 2, ficolin 2, ficolin B, serum lectin p35

Recovery

The recovery of human MX1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	98	90-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MX1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-89
1:2	Average %	94
	Range %	91-99
1:4	Average %	95
	Range %	92-98
1:8	Average %	106
	Range %	98-110

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	1.918	1.802	1.860	1.769
500	1.226	1.156	1.191	1.100
250	0.694	0.664	0.679	0.588
125	0.412	0.398	0.405	0.314
62.5	0.255	0.250	0.253	0.162
31.25	0.173	0.169	0.171	0.080
15.6	0.123	0.122	0.123	0.032
0	0.091	0.090	0.091	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q15485

Applications	ELISA
Research Area	FCN2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10502405	CSB-EL008551HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.