Human Fibulin 5 - FBLN5 ELISA Kit

Mfr.No.

E2109Hu

Description

This sandwich kit is for the accurate quantitative detection of Human Fibulin 5 (also known as IFN FBLN5) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Dance;Developmental arteries and neural crest EGF-like protein;FBLN 5;FBLN5;FIBL-5;Fibulin-5;UP50;Urine p50 protein

Reactivity

Human

Detection Range

30-9000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

50180

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

15.86ng/L

Background

Essential for elastic fiber formation, is involved in the assembly of continuous elastin (ELN) polymer and promotes the interaction of microfibrils and ELN (PubMed: 18185537). Stabilizes and organizes elastic fibers in the skin, lung and vasculature (By similarity). Promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. Vascular ligand for integrin receptors which may play a role in vascular development and remodeling (PubMed: 10428823). May act as an adapter that mediates the interaction between FBN1 and ELN (PubMed: 17255108).

Gene ID	10516
UniProt ID	Q9UBX5
Antigen	FBLN5

Research Area

Signal Transduction

Protocol

These standard curves of E2109Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4800ng/L	2.164	2.178	2.13
	2.191
2400ng/L	1.283	1.308	1.26
	1.333
1200ng/L	0.78	0.828	0.781
	0.877
600ng/L	0.517	0.507	0.459
	0.497
300ng/L	0.308	0.265	0.217
	0.222
0ng/L	0.052	0.048	0
	0.043

Precision

We measured random samples of E2109Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	917.8	66.84	7.3
Intra-Assay	2	18	3209.5	141.93	4.4
Intra-Assay	3	18	1568.5	82.62	5.3

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103656	E2109Hu	48T	$320		Add to Cart
BHE12103656	E2109Hu	96T	$458		Add to Cart

Human Fibulin 5 - FBLN5 ELISA Kit

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