Human Fibulin-5(FBLN5) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL008454HU

Alternative Names

fibulin 5, ARMD3, DANCE, EVEC, FIBL-5, FLJ90059, UP50, developmental arteries and neural crest epidermal growth factor-like, urine p50 protein

Reactivity

Human

Detection Range

62.5 pg/mL-4000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human MUC4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-98
EDTA plasma (n=4)	88	82-94

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MUC4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	89-96
1:2	Average %	103
	Range %	98-107
1:4	Average %	95
	Range %	89-99
1:8	Average %	93
	Range %	86-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

15.6 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q9UBX5
Antigen	Fibulin 5

Applications

ELISA

Research Area

FBLN5

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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