Human Fibronectin Type 3 Domain-containing Protein 5

Mfr.No.

E3206Hu

Sensitivity

2.43ng/mL

Detection Range

5-1000ng/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Fibronectin type III domain-containing protein 5;Fibronectin type III repeat-containing protein 2;FNDC 5;FNDC5;Irisin

Molecular Weight

23659

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E3206Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	227.5	9.02	4
Intra-Assay	2	18	84.5	5.49	6.5
Intra-Assay	3	18	109.7	5.31	4.8

Background

(Irisin): Contrary to mouse, may not be involved in the beneficial effects of muscular exercise, nor in the induction of browning of human white adipose tissue.

Protocol

These standard curves of E3206Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
640ng/mL	2.278	2.325	2.291
	2.371
320ng/mL	1.953	1.911	1.878
	1.869
160ng/mL	1.131	1.218	1.185
	1.306
80ng/mL	0.603	0.642	0.608
	0.681
40ng/mL	0.434	0.414	0.38
	0.393
0ng/mL	0.032	0.033	0
	0.035

Gene ID	252995
UniProt ID	Q8NAU1

Research Area	Metabolism

Storage	2-8°C

Reference	Oral ?-lipoic acid supplementation in patients with non-alcoholic fatty liver disease: effects on adipokines and liver histology features Journal: Food Funct. 2019 Aug 1;10(8):4941-4952

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104735	E3206Hu	48T	$320		Add to Cart
BHE12104735	E3206Hu	96T	$458		Add to Cart

Human Fibronectin Type 3 Domain-containing Protein 5 - FNDC5 ELISA Kit

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